Vitrification Cryotop®

Kitazato is globally recognised as one of the pioneering brands in driving and improving vitrification. Its greatest contribution in this field has been the development of the renowned Cryotop® Method, the global leader in vitrification of oocytes and embryos, in all stages of development.

The Cryotop® Method is available in 2 versions: vitrification in an open system and a closed system

  • Cryotop® – Open System.

    The original device, globally renowned for its unparalleled Cooling and Warming Rates and its very high survival rates.

  • Cryotop® SC – Closed System.

    The same results and the same protocol with a new sealing for achieving vitrification without direct contact of the specimen with the liquid nitrogen.

CRYOTOP® Closed System

Cryotop® SC continues to obtain the same excellent results as its Open System version. Once again Kitazato offers the best survival rates thanks to its unparalleled Cooling and Warming rates: -3000ºC/minute and 42000ºC/minute respectively.

 

CRYOTOP® Open System

Achieve the highest survival rates on the market with the Cryotop® Method, the global leader in vitrification of oocytes and embryos in all stages of development.

 

CRYOTOP® Closed System

Cryotop® SC continues to obtain the same excellent results as its Open System version. Once again Kitazato offers the best survival rates thanks to its unparalleled Cooling and Warming rates: -3000ºC/minute and 42000ºC/minute respectively.

 

Vitrification Warming Media

Kitazato media are the most versatile option for cryopreservation in your laboratory. Reduce your costs by using the same media for vitrification and warming of oocytes and embryos, in all their stages of development, from Pronucleus to Blastocyst.

VITROMEDia

VITROMEDia products are ready to use and with simple protocols, which will reduce work stress and time for embryologists.

V-GRAD

Gradient for Sperm Cell Isolation

  • Endotoxin tested
  • Sperm Survival Test ≥ 80 %
  • Colored with Phenol Red
  • Shelf life 18 months from date of production
  • V-GRAD is a sterile colloidal suspension of silica particles stabilized with covalently bound hydrophilic silanes
  • V-GRAD 100 % stock solution and ready-to-use versions
  • V-GRAD 40 % and V-GRAD 80 % diluted with HEPES buffered HTF
  • 100 mL PETG bottle and glass bottle
  • Kit: 2 x 12 mL 40 % and 80 % glass bottles each
 

V-HYADASE

For removing cumulus cells

V-HYADASE contains 80 IU/mL of Hyaluronidase in HEPES buffered HTF with HSA. For the removal of cumulus and corona cells from the oocyte prior to ICSI procedure.

  • Endotoxin tested (to the maximum detection limit of < 1 EU/mL)
  • MEA (1-cell to expanded blastocyst in 96 h) ≥ 80%
  • Packaging: 5 x 1 mL tube
  • Storage: 2-8 °C

V-PURE

CELL CULTURE WATER

This product is ideally suited for nuclear transfer, transfection, transformation, cell and tissue culture, LAL testing and all other aqueous solution preparation that require stringent quality control.

QUALITY CONTROL

  • Multiple phases of purified and 0.1μm filtered
  • LAL tested
  • Endotoxin-free (to the maximum detection limit of <0.005 EU/mL by turbidimetric assay)
  • Mouse Embryo Assay tested
  • Tested for use in critical assays and applications
  • Most sterile and stable packing

PACKAGING

2000 mL

V-PVP 7

FOR SPERM IMMOBILIZATION

V-PVP is a pharma grade Polyvinyl-Pyrrolidone (PVP) 7% in HEPES buffered HTF with HSA. For reducing the sperm motility and easy handling of sperms in ICSI procedure.

QUALITY CONTROL

  • Endotoxin tested (to the maximum detection limit of < 0.25 EU/mL)
  • MEA (1-cell to expanded blastocyst in 96 h) ≥ 80%

PACKAGING

  • 5 x 0.5 mL tube

STORAGE

  • 2-8 °C

V-ONESTEP

FOR SINGLE STEP CULTURE

V-ONESTEP is for continuous embryo culture from Fertilization to Blastocyst and embryo transfer.

QUALITY CONTROL

  • Endotoxin tested (to the maximum detection limit of < 0.25 EU/mL)
  • MEA (1-cell to expanded blastocyst in 96 h) ≥ 80%

PACKAGING

  • 20 mL glass vial

STORAGE

  • 2-8 °C

V-SPERM WASH

MEDIUM FOR
SPERM PREPARATION

V-SPERMWASH is a HEPES buffered HTF with HSA, intended for sperm washing and incubation for IUI, IVF and other assisted reproductive techniques.

QUALITY CONTROL

  • Endotoxin tested (to the maximum detection limit of < 0.25 EU/mL)
  • MEA (1-cell to expanded blastocyst in 96 h) ≥ 80%

PACKAGING

  • 100 mL PET bottle

STORAGE

  • 2-8 °C

V-HEPES PLUS

FOR MANIPULATION
OF GAMETES

V-HEPES PLUS is a HEPES buffered HTF with HSA. For IVF procedures which include the retrieval, handling and transfer of human gametes and embryos.

QUALITY CONTROL

  • Endotoxin tested (to the maximum detection limit of < 0.25 EU/mL)
  • MEA (1-cell to expanded blastocyst in 96 h) ≥ 80%

PACKAGING

  • 100 mL PET bottle

STORAGE

  • 2-8 °C

V-OIL

LIGHT PARAFFIN OIL

V-OIL is a ready to use light paraffin oil for ART-procedures and applicable for short and long term cultures up to 6 days. QA leaflet here!

QUALITY CONTROL

  • Endotoxin and Peroxide tested
  • 120h Mouse Embryo Assay with additional cell count
  • Prewashed twice with high purity water
  • Shelf life 18 months from date of production

PACKAGING

  • 100 and 500 mL PETG or glass bottle

STORAGE

  • 2-8 °C

V-SPERM FREEZE

FOR SPERM CRYOPRESERVATION

V-SPERMFREEZE is a HEPES buffered cryoprotectant medium for cryopreservation and storage of human sperm cells.

QUALITY CONTROL

  • Endotoxin tested (to the maximum detection limit of < 0.25 EU/mL)
  • MEA (1-cell to expanded blastocyst in 96 h) ≥ 80%

PACKAGING

  • 4 x 12 ml glass vial

STORAGE

  • 2-8 °C